Products

The SPOT-LX™ Platform
Functional Multiplex Repair assays

LXRepair's experts have developed the SPOT-LX™ product range, comprising 3 different assays:
  • Glyco-SPOT: quantification or Glycosylase and AP endonuclease activity
    (from Base Excision Repair pathway),
  • ExSy-SPOT: quantification of Excision/Synthesis Repair activities
    (from Base Excision Repair, Nucleotide Excision Repair and Intra Cross-Link repair pathways),
  • Next-SPOT: quantification of Double-Strand Break Repair
    (validation ongoing)
LXRepair's member using scanner

Principle

All assays are based on the same principle.
Series of DNA substrates containing specific lesions are immobilized on biochips. Extracts prepared from the samples to be characterized are applied on the biochip. The sample's DNA Repair enzymes repair their different substrates and generate fluorescent changes on the biochip yielding a specific Signature for each sample.

Fluorescence is quantified using a scanner.

Assay characteristics

Standardized, Fast, Powerful assays on biochip

Development of the DNA Repair assays on biochips (standard glass slide format) compares favorably to traditional DNA Repair assays:
  • Standardized and controlled manufacturing processes
  • Positive controls included on-chip
  • Internal quality controls for the repair reaction
  • Standardized data analysis processes
  • Assays are fast and easy-to-use

Multiplexing:
analyzing multiple targets in a single reaction volume

Multiplexing allows the simultaneous analysis of several DNA Repair activities in parallel.
It has several advantages:
  • Saves times
  • Uses limited sample volumes
  • Increases sample throughput

Characterizing the whole DNA repair network

These assays provide an overview of most DNA repair activities. Activated/ inhibited DNA repair pathways are identified.
Combined with bioinformatic analysis, the SPOT-LX™ platform provides a powerful method to gain insights into DNA Repair regulation by upstream signaling networks and to identify the drivers activating specific pathways and the functional consequences of mutations in DNA repair and DDR genes.

Suitable Samples

Preclinical and clinical models

LXRepair assays can characterize DNA repair activities in most living tissues or cells.

DNA Repair activities are measurable in any cell- or tissue-type, provided live cells are present. The DNA Repair profile varies depending on the cell type and the tissue of origin.
DNA Repair activities can also be characterized in tumor biopsies, 3D models, organoids.

Cell and tissue extracts

Extract preparation can be adapted depending on the amount of sample available (biopsy size, number of cells)
Nuclear or whole-cell extracts can be used.
DNA Repair activities are measurable in as few as 20.000 cells (carcinoma cell line).
Assays description

ExSy-SPOT

Multiplex Excision/Synthesis Repair Assay

ExSy-SPOT, Multiplex Excision/Synthesis Repair Assay
Lesions present on the biochip
8oxoG
AP sites
EthenoA
Glycols
}
BER
Photoproducts
Cisplatin Adducts
}
NER
ICLR
The very versatile assay quantifies excision/synthesis repair on a range of well-characterized lesions. 
Several versions of the assay exist to specifically investigate the following:
  • excision/synthesis repair
  • excision
  • polymerase efficiency and fidelity

Applications

  • Investigation of DNA Repair and DNA Damage Response
  • Functional network characterization
  • Impact of genotoxic compounds
  • DNA Repair inhibitors' mechanisms of action
  • Polymerase properties
  • DNA Repair inhibitor characterization
  • DNA Repair inhibitor screening

Glyco-SPOT

Multiplex oligonucleotide Cleavage Assay

Glyco-SPOT, Multiplex oligonucleotlde Cleavage Assay
The assay reveals the cleavage efficiency of Glycosylases/AP endonucleases
Lesions present on the biochip
8oxoG/C (OGG1)
A/8oxoG (MYH)
Hypoxanthine/T (AAG/MPG)
Thymine glycol /A (NTH – NEIL)
U/A (UNG)
U/G (SMUG)
EthenoA/T (AAG/MPG)
Abasic site (APE1)
}
BER
The assay monitors Glycosylase/AP endonuclease activity at basal level and in response to various stress factors.

Applications

  • Characterization of DNA Repair inhibitors
  • Screening DNA Repair inhibitors
  • Analysis of defenses against oxidative stress
  • Inflammation studies
  • Aging studies
  • Investigation of neutraceutical properties

Ordering information:

Please follow the link to our manufacturing partner and distributor Bertin Pharma to purchase our first RUO assay on the market:

Next-SPOT

Repair pathway choice has a decisive influence on a patient’s response to cancer treatment. Our goal is to develop Next-SPOT, an enzymatic assay capable of characterizing the cells’ DSB repair capacity while providing information on the predominant pathway involved, in order to better predict a patient’s response to ionizing radiations.
Service

Service

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Biomarker identification

We provide biomarker research as a paying service.
LXRepair can run DNA Repair assays using customer's own sample materials (cells, biopsies) and provide the original data as well as detailed reports summarizing the results. We help you design the experiments and interpret the results.

Mentoring is provided for biotech and pharmaceutical companies for their drug development programs, from preclinical to clinical stages.
Our experienced laboratory engineers and scientific experts will work closely with you to determine your specific needs and develop customized solutions.

On request, specific protocols can be provided to help customers prepare appropriate extracts from their own sample materials.

Screening Assay Service

Screening assays can be set up using LXRepair technologies to help you to identify effective DNA repair inhibitors.
The Glyco-SPOT assay is directly transferable to external screening platforms.

Partnership

Partnership

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Diagnostic Development

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pharmaceutical industry industry

We are seeking strategic partners in the pharmaceutical or diagnostic industry to discover new biomarkers and develop diagnostic or companion diagnostic kits using our proprietary technologies.
Our first-in-class assays complement existing genomics profiling by providing functional information and allowing the impact of genomic modifications on protein functions to be assessed.

If you are interested in exploring a strategic partnership with us, we would be happy to hear from you.

R&D partnership

R&D partnership icon

As an SME, LXRepair is eligible for EU funding for innovative collaborative research projects.