Principle

LXRepair technology has been developed to establish the DNA Repair Enzyme Signature.

DNA Repair enzymes contained in a biological sample react with series of lesion-containing DNA on a biochip. The repair reaction occurring at each lesion site is measured by fluorescence analysis.

LXRepair technology combines:

  • A multiplexed approach enabling the simultaneous screening of several enzymatic DNA Repair activities from a single sample on a microsupport
  • A quantitative detection

LXRepair technology

  • Multiple detection
  • System vision
  • Comparative quantification of different DNA Repair pathways
Schematic representation of LXRepair technology on enzymatic DNA Repair activities

Traditional approach

  • Single detection
Schematic representation of traditional approach of technology on enzymatic DNA Repair activities compared to LXRepair technology

Publications

  • A comprehensive approach to determining BER capacities and their change with aging in Drosophila melanogaster mitochondria by oligonucleotide microarray. Garreau-Ballandier I et al. FEBS Lett. 2014; 588:1673-9.
  • Alzheimer's disease-associated neurotoxic Peptide amyloid-β impairs base excision repair in human neuroblastoma cells. Forestier A et al. Int J Mol Sci. 2012; 13:14766-87.
  • Functional DNA repair signature of cancer cell lines exposed to a set of cytotoxic anticancer drugs using a multiplexed enzymatic repair assay on biochip. Forestier A et al, PLoS One. 2012; 7:e51754.
  • Titanium dioxide nanoparticles exhibit genotoxicity and impair DNA repair activity inA549 cells. Jugan ML et al. Nanotoxicology. 2012; 6:501-13.
  • Aging and photo-aging DNA repair phenotype of skin cells - Evidence toward an effect of chronic sun-exposure. Prunier C et al. Mutat Res. 2012; 736:48-55.
  • Age-associated modifications of Base Excision Repair activities in human skin fibroblast extracts. Pons B et al. Mech Ageing Dev. 2010; 131:661-5.
  • Effect of aging on DNA excision/synthesis repair capacities of human skin fibroblasts. Sauvaigo S et al. J Invest Dermatol. 2010; 130:1739-41.
  • A microarray to measure repair of damaged plasmids by cell lysates. Millau JF et al. Lab Chip. 2008; 8:1713-22.
  • Repair of oxidative damage of thymine by HeLa whole-cell extracts: simultaneous analysis using a microsupport and comparison with traditional PAGE analysis. Guerniou V et al. Biochimie. 2005; 87:151-9.
  • An oligonucleotide microarray for the monitoring of repair enzyme activity toward different DNA base damage. Sauvaigo S et al. Anal Biochem. 2004; 333:182-92.

With us reach the next level

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